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CHYTRID FUNGUS - SINGAPORE: FIRST REPORT
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A ProMED-mail post
ProMED-mail is a program of the
International Society for Infectious Diseases
Date: Sun 6 Mar 2012 [ProMED regrets the delay in posting]
Source: Sciencedaily.com [edited]
Deadly Fungus Detected in Southeast Asia's Amphibian Trade
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A team of scientists led by the Wildlife Conservation Society (WCS)
and the National University of Singapore (NUS), revealed in a new
study, for the 1st time, the presence of the pathogenic chytrid fungus
(_Batrachochytrium dendrobatidis_) in amphibians sampled in Singapore.
And the American bullfrog may be a central player in the spread of the
disease.
The study appears in the current issue of the journal EcoHealth, and
is the 1st to consider the role that Southeast Asia's commercial trade
plays in the spread of amphibian pathogens. Demand for amphibians
through local and international trade is high and fueled by use of
frogs as pets, food, bait, and as a source of traditional 'medicine.'
More than 40 percent of amphibian species are in decline globally due,
not only to chytrid fungus, but also overharvesting, competition from
invasive species, habitat loss, pollution, and climate change.
In the study, scientists collected samples from 2389 individual
animals in Lao PDR, Cambodia, Viet Nam, and Singapore at 51 different
sites including farms, locally supplied markets, pet stores, and from
the wild.
The molecular testing of samples was led by Dr. Tracie Seimon at WCS's
Molecular Diagnostic Laboratory at the Bronx Zoo. Results showed that
frogs from Lao PDR and Viet Nam tested negative for chytrid. In
Cambodia, one frog intended for food tested positive. In addition, 74
animals in Cambodia and Viet Nam were screened for ranavirus and
tested negative, suggesting that these specific pathogens are not yet
a conservation threat in species tested from these countries. In
Singapore, however, 13 samples tested positive for chytrid and
represent the 1st report of chytrid in the territory. Of those
samples, 11 were collected from 4 pet stores and the remaining 2 were
taken from amphibians in the wild.
The scientists noted that the chytrid detections were most prevalent
in the American bullfrog (_Lithobates (=Rana) catesbeiana_), a common
species in the trade and one that is tolerant of chytrid infections.
"Finding chytrid in 4 of the 7 Singaporean pet stores we sampled is
cause for concern," said lead author and WCS Scientist Martin Gilbert.
"Since the American bullfrog is able to tolerate this pathogen, it may
act as a carrier for spreading chytrid to the region when it is
imported through commercial trade."
In another alarming discovery, the scientists found that all 497 frogs
sampled from 23 frog farms in Viet Nam had skin lesions ranging from
swelling and inflammation to ulcers and deformed or missing digits in
the most severe cases. Disease examination revealed 4 of the animals
had bacteria associated with the lesions that in 2 cases appeared to
have spread to other organs.
While the bacteria and its role as primary or secondary pathogen could
not be positively identified, the scientists noted that frog farms
could serve as a source of infection for the wider environment.
The study noted that lesions among frogs raised at commercial
facilities in Viet Nam are of particular concern, in light of the low
level of bio-security that exists. All of the farms in the study
disposed of untreated wastewater directly into natural watercourses,
which becomes an avenue to spread infection to other places and other
species.
According to the authors, the World Organization for Animal Health
(OIE) requires its 174 member countries, which include the 4 countries
in this study, to conduct surveillance for chytrid fungus, report
confirmed cases, and implement measures to control its spread.
Co-author of the study, Assistant Professor David Bickford from the
Department of Biological Sciences at the NUS [National University of
Singapore] Faculty of Science, said, "In light of the fact that this
emerging infectious disease is now known to be spread by commercial
trade, it is in everyone's best interest to eliminate it from the
trade in live animals before both the native amphibian populations of
Southeast Asia are affected and before it completely decimates the
commercial trade and people are unable to make a living. This is not
just about the frogs."
The paper concludes, "There is an urgent need to conduct wider surveys
of wild amphibians in Southeast Asia to determine the extent and
severity of chytrid fungus and other infectious diseases among a range
of species, and whether and how these change over time. Studies should
focus on differentiating _Batrachochytrium dendrobatidis_ strains that
may be endemic to the region from exotic strains that may be
introduced through routes including international trade."
Authors of the study include Martin Gilbert of WCS; David Bickford of
NUS; Leanne Clark, Arlyne Johnson, Priscilla H. Joyner, Lucy Ogg
Keatts, Kongsy Khammavong, Long Nguyen Van, Alisa Newton of WCS;
Tiffany P. W. Seow of NUS; Scott Roberton, Soubanh Silithammavong of
WCS; Sinpakhone Singhalath of the National University of Laos; Angela
Yang, and Tracie A. Seimon of WCS.
[Byline: Rick Steelhammer]
--
Communicated by:
ProMED-mail from HealthMap Alerts
[This discovery highlights the potential importance of wildlife trade
in the global spread of a pathogen.
_B. dendrobatidis_ (chytrid fungus) is a singular fungal pathogen. It
has a swimming zoospore as the infectious stage. It colonizes skin
cells of adult amphibians and the mouthparts of tadpoles. Infection
occurs when zoospores land on the skin or the mouthparts and encyst.
Through mechanisms that are not understood, the pathogen moves from
the surface of the skin to the stratum granulosum of the epidermis and
matures in the stratum corneum. There, it enters healthy cells, grows
in size, and develops into an urn-shaped zoosporangium in which the
zoospores develop. As infected skin cells move toward the surface, the
zoosporangium matures, the discharge papilla opens, and mature
zoospores swim out. The mechanism by which this pathogen causes
disease seems to be by interference with the ion transport functions
of the skin, leading eventually to cardiac failure.
Worldwide amphibian decline has been related to epidemics caused by
the chytrid fungus. The finding reported here becomes really relevant,
as one of the hypotheses to explain the global amphibian decline is
that the chytrid fungus acts as a propagating pathogen that was taken
out of Africa by wildlife trade of _Xenopus_ spp. However, evidence
indicates that this fungus is not the single cause of the worldwide
amphibian decline (it is not necessary nor sufficient to cause
population declines). Prevalences of various pathogens (other than
chytrid fungus) were greater in declining amphibian populations than
in non-declining ones (an indication that pathogens are affecting
amphibian populations), as it is also reported here.
It has been shown that global warming can degrade toads' condition and
that frog declines caused by the chytrid fungus were preceded by
periods of increasing stress (an indication that poor condition is
implicated in population declines). In addition, it was found that the
severity of the infection by chytrid depends on the vigour of the
individual infected. Moreover, amphibians also decline in areas where
the fungus is absent, and the fungus is sometimes present for long
periods of time without causing disease. Thus, an impoverished
condition resulting from environmental changes may be triggering
vicious cycles (in which the chytrid fungus may be implicated) that
are decimating amphibian populations. - Mod.PMB
A HealthMap/ProMED-mail map can be accessed at:
.]
[see also:
2012
----
Chytrid fungus, crayfish - USA: non-amphibian hosts 20130105.1483017
2011
----
Chytrid fungus, frogs - worldwide: mechanism of spread
2010
----
Chytrid fungus, frogs - Worldwide: possible recovery 20101212.4421
Chytrid fungus, frogs - worldwide: review article 20100130.0323
2009
----
Chytrid fungus, frog - South Korea 20090920.3301
Chytrid fungus, frog - Philippines: (Luzon) 20090527.1976
2008
----
Chytrid fungus, frogs - Panama 20081014.3246
Chytrid fungus, frogs - Spain (Majorca) 20080928.3065
2007
----
Chytrid fungus, frogs - Japan (02): wild frogs 20070613.1924
Chytrid fungus, frogs - Japan 20070113.0176
2006
----
Chytrid fungus, frogs - worldwide: possible source 20060524.1463
Chytrid fungus, frogs - South Africa 20060203.0344
2005
----
Chytrid fungus, frogs - UK (England) 20050916.2741
2004
----
Red leg disease, frogs, fatal - UK (02) 20040914.2560
Red leg disease, frogs, fatal - UK 20040912.2542
2002
----
Frog deformities - USA (02) 20020425.4030
Frog deformities - USA 20020422.4012
Frog mortality, virus - UK 20020201.3458
2000
----
Chytrid fungus, frogs: background 20001201.2096
Frog deformities - USA (Northeast) 20000420.0579]
.................................................sb/pmb/ejp/jw
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